Audio Effects unit on zynthian 4

OK, I’m having some trouble understanding what you said, but, I think you might be helped by this thread:

particularly riban’s last post from August 4 2021, which starts with:
By default the audio inputs are not connected to the outputs. You must insert at least one audio effect which will connect the inputs and outputs. You can change routing in the layer’s option menu. Check out the Layers section of the user manual .

and goes on to provide more details on levels and bass guitar input.